Part:BBa_K4628021:Design

TMV RNA dependent RNA polymerase

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal XbaI site found at 987
  Illegal XbaI site found at 3347
  Illegal SpeI site found at 1206
  Illegal SpeI site found at 3209
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal NheI site found at 1910
  Illegal SpeI site found at 1206
  Illegal SpeI site found at 3209
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal BglII site found at 2609
  Illegal BglII site found at 3344
  Illegal BglII site found at 4172
  Illegal BglII site found at 4267
  Illegal BamHI site found at 3320
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal XbaI site found at 987
  Illegal XbaI site found at 3347
  Illegal SpeI site found at 1206
  Illegal SpeI site found at 3209
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal XbaI site found at 987
  Illegal XbaI site found at 3347
  Illegal SpeI site found at 1206
  Illegal SpeI site found at 3209
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4726
  

Design Notes

Considering that TMV is a mature carrier in plant chassis, we believe that TMV RNA-dependent RNA polymerase should also be able to copy and transcribe specific RNA in plants. Therefore, we selected TMV RNA-dependent RNA polymerase as a functional protein that extends the half-life of a specific RNA.

Source

Tobacco mosaic virus genome (NCBI Reference Sequence: NC_001367.1)